Introduction of ultrasonic extraction of antler polypeptide

Introduction of ultrasonic extraction of antler polypeptide

First, the introduction of pilose peptide

According to the "Compendium of Materia Medica" in China, the antler has the functions of tonifying the kidney and strengthening the yang, strengthening the bones and strengthening the bones, and promoting the essence and benefiting blood. Velvet antler is a special cartilage tissue containing a variety of active factors. Modern medicine shows that the chemical composition of velvet antler is complex, which contains a large number of amino acids, peptides, proteins, inorganic elements and other components, and the protein component is as high as 55.26%, which proves that antler polypeptide is its main biological active ingredient.

Velvet antler polypeptide is a small molecule protein with a molecular weight of 1,000 to 50,000, which is strong in activity, easy to absorb, and has little side effects. Studies have shown that pilose antler polypeptide has anti-inflammatory, anti-oxidant, immune-enhancing and can promote cell differentiation, wound healing, inhibit tumor cells, promote the regeneration of the nervous system, peripheral nervous system and other pharmacological effects, so the research on velvet antler polypeptide is more and more Significance. How to extract velvet antler polypeptide efficiently is a necessary prerequisite for the study of velvet antler polypeptide.

Second, the common extraction method of velvet antler polypeptide

1.Organic solvent extraction

The organic solvent extractant is used to extract the protein and polypeptide active ingredients in the velvet antler using the extract having different partition coefficients in the two mutually 2.incompatible reagents.

Lysis method

A method of rapidly breaking cell tissue by a lysate.

3. Enzymatic hydrolysis

Enzymatic hydrolysis is the use of active enzymes to hydrolyze specific substances. In the experiment, effective extraction is carried out by controlling the type of the enzyme, the time and temperature of the reaction, and the hydrogen ion concentration index.

4, homogenization method

The sample containing a certain amount of water and the extraction solvent are mixed and homogenized in the homogenization extraction device, and the material is torn and pulverized by mechanical and hydraulic shearing, so that the material is broken and the extraction of the active ingredient is synchronously carried out, thereby realizing the rapid active ingredient. And strengthen the purpose of extraction.

5, ultrasonic extraction

Ultrasonic extraction uses ultrasonic extraction machine to increase the molecular motion frequency of substances by using the strong cavitation effect, mechanical vibration, disturbance effect, high acceleration, emulsification, diffusion, crushing and stirring effects generated by ultrasonic radiation pressure. And speed, increasing solvent penetration, thereby accelerating the target component into the solvent, facilitating the extraction of mature extraction techniques

third. Comparative study of homogenization method and ultrasonic extraction method

result

According to the statistical method, the data of each of the above homogenization method and the ultrasonic extraction method were measured three times, and the average value was calculated to obtain SD (relative standard deviation) of three data.

The experimental results are very obvious. The concentration of velvet antler treated by ultrasonic extraction reached 1.25mg/mL at 20mins, while the concentration increased from 40mins to 80mins, which almost reached the highest value.

The homogenate method was used to measure the protein concentration results (such as the chart). The concentration of velvet antler protein increased slowly with the increase of homogenization time. When homogenate for 6 h, the concentration of velvet antler protein was only 0.42 mg/mL.

fourth. Extraction of antler polypeptide by ultrasonic extraction

The above experiments show that the extraction of antler polypeptide by ultrasonic extraction has advantages.

(1)     Saving time and high efficiency. The extraction efficiency in one hour is more than 10 times that of the homogenization method, and the relative acquisition rate is the highest when the ultrasonic extraction time is 40 minutes.

(2) The extraction yield is high. Compared with the homogenization method, the ultrasonic stabilization period extraction yield is about 4 times that of the homogenization method.